Analysis of glucose-protein interaction using <i>p</i>-hydroxyacetophenone-Sepharose affinity resin —Glucose decreases alcohol dehydrogenase activity <i>in vitro</i>—
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منابع مشابه
Purification of Liver Aldehyde Dehydrogenase by p- Hydroxyacetophenone-Sepharose Affinity Matrix and the Coelution of Chloramphenicol Acetyl Transferase Recombinantly Expressed from the Same Matrix with Aldehyde Dehydrogenase’
p-Hydroxyacetophenone was coupled to epoxy-activated Sepharose 6B to generate an afflnity chromatographic matrix to purify aldehyde dehydrogenase. Purified beef liver mitochondrial aldehyde dehydrogenase specifically bound to the support and could be eluted with phydroxyacetophenone. A post-ammonium sulfate (3045%) fraction of bovine liver was applied to the affinity gel column and aldehyde deh...
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ژورنال
عنوان ژورنال: Journal of Electrophoresis
سال: 2012
ISSN: 1349-9394,1349-9408
DOI: 10.2198/jelectroph.56.19